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Fig. 8. Effect of ONO-2506 administration on glial fibrillary acidic protein expression in rat brain striatum. (A–C) Western blotting results showed that compared with that in the saline group, the expression of glial fibrillary acidic protein <t>(GFAP)</t> in the right striatum of the ONO-2506 group was significantly increased (day 4: ONO-2506 group saline group ± standard error of mean [SEM]: 319.7% ± 42.10%, p < 0.0001, t = 7.594, df = 16; day 8: ONO-2506 group saline group ± SEM: 563.4% ± 143.5%, p = 0.0012, t = 3.926, df = 16). (D–G) Representative images of GFAP immunohistochemical staining in the ONO-2506 and saline groups. (H) Immunohistochemical staining showed that the level of GFAP in the right striatum of rats in the ONO-2506 group was higher than that in the right side of saline group (ONO-2506 group saline group ± SEM: 137.4% ± 30.08%, p = 0.0103, t = 4.568, df = 4) and was higher than that in the left striatum (ONO-2506 group right left ± SEM: 147.6% ± 34.13%, p = 0.0496, t = 4.324, df = 2). * p < 0.05; **p < 0.01.
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Fig. 8. Effect of ONO-2506 administration on glial fibrillary acidic protein expression in rat brain striatum. (A–C) Western blotting results showed that compared with that in the saline group, the expression of glial fibrillary acidic protein <t>(GFAP)</t> in the right striatum of the ONO-2506 group was significantly increased (day 4: ONO-2506 group saline group ± standard error of mean [SEM]: 319.7% ± 42.10%, p < 0.0001, t = 7.594, df = 16; day 8: ONO-2506 group saline group ± SEM: 563.4% ± 143.5%, p = 0.0012, t = 3.926, df = 16). (D–G) Representative images of GFAP immunohistochemical staining in the ONO-2506 and saline groups. (H) Immunohistochemical staining showed that the level of GFAP in the right striatum of rats in the ONO-2506 group was higher than that in the right side of saline group (ONO-2506 group saline group ± SEM: 137.4% ± 30.08%, p = 0.0103, t = 4.568, df = 4) and was higher than that in the left striatum (ONO-2506 group right left ± SEM: 147.6% ± 34.13%, p = 0.0496, t = 4.324, df = 2). * p < 0.05; **p < 0.01.
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Fig. 8. Effect of ONO-2506 administration on glial fibrillary acidic protein expression in rat brain striatum. (A–C) Western blotting results showed that compared with that in the saline group, the expression of glial fibrillary acidic protein <t>(GFAP)</t> in the right striatum of the ONO-2506 group was significantly increased (day 4: ONO-2506 group saline group ± standard error of mean [SEM]: 319.7% ± 42.10%, p < 0.0001, t = 7.594, df = 16; day 8: ONO-2506 group saline group ± SEM: 563.4% ± 143.5%, p = 0.0012, t = 3.926, df = 16). (D–G) Representative images of GFAP immunohistochemical staining in the ONO-2506 and saline groups. (H) Immunohistochemical staining showed that the level of GFAP in the right striatum of rats in the ONO-2506 group was higher than that in the right side of saline group (ONO-2506 group saline group ± SEM: 137.4% ± 30.08%, p = 0.0103, t = 4.568, df = 4) and was higher than that in the left striatum (ONO-2506 group right left ± SEM: 147.6% ± 34.13%, p = 0.0496, t = 4.324, df = 2). * p < 0.05; **p < 0.01.
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Fig. 8. Effect of ONO-2506 administration on glial fibrillary acidic protein expression in rat brain striatum. (A–C) Western blotting results showed that compared with that in the saline group, the expression of glial fibrillary acidic protein <t>(GFAP)</t> in the right striatum of the ONO-2506 group was significantly increased (day 4: ONO-2506 group saline group ± standard error of mean [SEM]: 319.7% ± 42.10%, p < 0.0001, t = 7.594, df = 16; day 8: ONO-2506 group saline group ± SEM: 563.4% ± 143.5%, p = 0.0012, t = 3.926, df = 16). (D–G) Representative images of GFAP immunohistochemical staining in the ONO-2506 and saline groups. (H) Immunohistochemical staining showed that the level of GFAP in the right striatum of rats in the ONO-2506 group was higher than that in the right side of saline group (ONO-2506 group saline group ± SEM: 137.4% ± 30.08%, p = 0.0103, t = 4.568, df = 4) and was higher than that in the left striatum (ONO-2506 group right left ± SEM: 147.6% ± 34.13%, p = 0.0496, t = 4.324, df = 2). * p < 0.05; **p < 0.01.
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Fig. 8. Effect of ONO-2506 administration on glial fibrillary acidic protein expression in rat brain striatum. (A–C) Western blotting results showed that compared with that in the saline group, the expression of glial fibrillary acidic protein <t>(GFAP)</t> in the right striatum of the ONO-2506 group was significantly increased (day 4: ONO-2506 group saline group ± standard error of mean [SEM]: 319.7% ± 42.10%, p < 0.0001, t = 7.594, df = 16; day 8: ONO-2506 group saline group ± SEM: 563.4% ± 143.5%, p = 0.0012, t = 3.926, df = 16). (D–G) Representative images of GFAP immunohistochemical staining in the ONO-2506 and saline groups. (H) Immunohistochemical staining showed that the level of GFAP in the right striatum of rats in the ONO-2506 group was higher than that in the right side of saline group (ONO-2506 group saline group ± SEM: 137.4% ± 30.08%, p = 0.0103, t = 4.568, df = 4) and was higher than that in the left striatum (ONO-2506 group right left ± SEM: 147.6% ± 34.13%, p = 0.0496, t = 4.324, df = 2). * p < 0.05; **p < 0.01.
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Fig. 8. Effect of ONO-2506 administration on glial fibrillary acidic protein expression in rat brain striatum. (A–C) Western blotting results showed that compared with that in the saline group, the expression of glial fibrillary acidic protein <t>(GFAP)</t> in the right striatum of the ONO-2506 group was significantly increased (day 4: ONO-2506 group saline group ± standard error of mean [SEM]: 319.7% ± 42.10%, p < 0.0001, t = 7.594, df = 16; day 8: ONO-2506 group saline group ± SEM: 563.4% ± 143.5%, p = 0.0012, t = 3.926, df = 16). (D–G) Representative images of GFAP immunohistochemical staining in the ONO-2506 and saline groups. (H) Immunohistochemical staining showed that the level of GFAP in the right striatum of rats in the ONO-2506 group was higher than that in the right side of saline group (ONO-2506 group saline group ± SEM: 137.4% ± 30.08%, p = 0.0103, t = 4.568, df = 4) and was higher than that in the left striatum (ONO-2506 group right left ± SEM: 147.6% ± 34.13%, p = 0.0496, t = 4.324, df = 2). * p < 0.05; **p < 0.01.
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Fig. 8. Effect of ONO-2506 administration on glial fibrillary acidic protein expression in rat brain striatum. (A–C) Western blotting results showed that compared with that in the saline group, the expression of glial fibrillary acidic protein <t>(GFAP)</t> in the right striatum of the ONO-2506 group was significantly increased (day 4: ONO-2506 group saline group ± standard error of mean [SEM]: 319.7% ± 42.10%, p < 0.0001, t = 7.594, df = 16; day 8: ONO-2506 group saline group ± SEM: 563.4% ± 143.5%, p = 0.0012, t = 3.926, df = 16). (D–G) Representative images of GFAP immunohistochemical staining in the ONO-2506 and saline groups. (H) Immunohistochemical staining showed that the level of GFAP in the right striatum of rats in the ONO-2506 group was higher than that in the right side of saline group (ONO-2506 group saline group ± SEM: 137.4% ± 30.08%, p = 0.0103, t = 4.568, df = 4) and was higher than that in the left striatum (ONO-2506 group right left ± SEM: 147.6% ± 34.13%, p = 0.0496, t = 4.324, df = 2). * p < 0.05; **p < 0.01.
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Fig. 8. Effect of ONO-2506 administration on glial fibrillary acidic protein expression in rat brain striatum. (A–C) Western blotting results showed that compared with that in the saline group, the expression of glial fibrillary acidic protein <t>(GFAP)</t> in the right striatum of the ONO-2506 group was significantly increased (day 4: ONO-2506 group saline group ± standard error of mean [SEM]: 319.7% ± 42.10%, p < 0.0001, t = 7.594, df = 16; day 8: ONO-2506 group saline group ± SEM: 563.4% ± 143.5%, p = 0.0012, t = 3.926, df = 16). (D–G) Representative images of GFAP immunohistochemical staining in the ONO-2506 and saline groups. (H) Immunohistochemical staining showed that the level of GFAP in the right striatum of rats in the ONO-2506 group was higher than that in the right side of saline group (ONO-2506 group saline group ± SEM: 137.4% ± 30.08%, p = 0.0103, t = 4.568, df = 4) and was higher than that in the left striatum (ONO-2506 group right left ± SEM: 147.6% ± 34.13%, p = 0.0496, t = 4.324, df = 2). * p < 0.05; **p < 0.01.
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Fig. 8. Effect of ONO-2506 administration on glial fibrillary acidic protein expression in rat brain striatum. (A–C) Western blotting results showed that compared with that in the saline group, the expression of glial fibrillary acidic protein <t>(GFAP)</t> in the right striatum of the ONO-2506 group was significantly increased (day 4: ONO-2506 group saline group ± standard error of mean [SEM]: 319.7% ± 42.10%, p < 0.0001, t = 7.594, df = 16; day 8: ONO-2506 group saline group ± SEM: 563.4% ± 143.5%, p = 0.0012, t = 3.926, df = 16). (D–G) Representative images of GFAP immunohistochemical staining in the ONO-2506 and saline groups. (H) Immunohistochemical staining showed that the level of GFAP in the right striatum of rats in the ONO-2506 group was higher than that in the right side of saline group (ONO-2506 group saline group ± SEM: 137.4% ± 30.08%, p = 0.0103, t = 4.568, df = 4) and was higher than that in the left striatum (ONO-2506 group right left ± SEM: 147.6% ± 34.13%, p = 0.0496, t = 4.324, df = 2). * p < 0.05; **p < 0.01.
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Image Search Results


Fig. 8. Effect of ONO-2506 administration on glial fibrillary acidic protein expression in rat brain striatum. (A–C) Western blotting results showed that compared with that in the saline group, the expression of glial fibrillary acidic protein (GFAP) in the right striatum of the ONO-2506 group was significantly increased (day 4: ONO-2506 group saline group ± standard error of mean [SEM]: 319.7% ± 42.10%, p < 0.0001, t = 7.594, df = 16; day 8: ONO-2506 group saline group ± SEM: 563.4% ± 143.5%, p = 0.0012, t = 3.926, df = 16). (D–G) Representative images of GFAP immunohistochemical staining in the ONO-2506 and saline groups. (H) Immunohistochemical staining showed that the level of GFAP in the right striatum of rats in the ONO-2506 group was higher than that in the right side of saline group (ONO-2506 group saline group ± SEM: 137.4% ± 30.08%, p = 0.0103, t = 4.568, df = 4) and was higher than that in the left striatum (ONO-2506 group right left ± SEM: 147.6% ± 34.13%, p = 0.0496, t = 4.324, df = 2). * p < 0.05; **p < 0.01.

Journal: Neuroscience

Article Title: ONO-2506 Can Delay Levodopa-induced Dyskinesia in the Early Stage.

doi: 10.1016/j.neuroscience.2023.02.004

Figure Lengend Snippet: Fig. 8. Effect of ONO-2506 administration on glial fibrillary acidic protein expression in rat brain striatum. (A–C) Western blotting results showed that compared with that in the saline group, the expression of glial fibrillary acidic protein (GFAP) in the right striatum of the ONO-2506 group was significantly increased (day 4: ONO-2506 group saline group ± standard error of mean [SEM]: 319.7% ± 42.10%, p < 0.0001, t = 7.594, df = 16; day 8: ONO-2506 group saline group ± SEM: 563.4% ± 143.5%, p = 0.0012, t = 3.926, df = 16). (D–G) Representative images of GFAP immunohistochemical staining in the ONO-2506 and saline groups. (H) Immunohistochemical staining showed that the level of GFAP in the right striatum of rats in the ONO-2506 group was higher than that in the right side of saline group (ONO-2506 group saline group ± SEM: 137.4% ± 30.08%, p = 0.0103, t = 4.568, df = 4) and was higher than that in the left striatum (ONO-2506 group right left ± SEM: 147.6% ± 34.13%, p = 0.0496, t = 4.324, df = 2). * p < 0.05; **p < 0.01.

Article Snippet: Target Species Dilution ratio Supplier Article No. TH Rabbit 1:200 Proteintech 25859–1- AP GFAP Mouse 1:1,000 Servicebio GB12096 EAAT2 Rabbit 1:150 Proteintech 22515–1- AP TNFa Rabbit 1:200 Proteintech 17590–1- AP VEGF Rabbit 1:300 Santa Cruz Sc-7296 Iba-1 Rabbit 1:1,000 Proteintech 10904–1- AP

Techniques: Expressing, Western Blot, Saline, Immunohistochemical staining, Staining